MALDI-TOF has proven to perform as an accurate and cost-effective way to identify microbial samples. Our advanced utilization of MALDI-TOF enables us to accurately detect various bacterial and yeast species within each sample. Whether environmental monitoring, identification services, pinpointing specific contaminants in beverages like beer and wine, or swiftly identifying unknown substances in food or water products, MALDI-TOF delivers rapid and reliable results.
MALDI-TOF, short for Matrix Assisted Laser Desorption Ionization – Time of Flight, revolutionizes the identification process. Through the application of an energy-absorbing matrix (HCCA — 𝛂-Cyano-4-hydroxycinnamic acid in our case), the sample undergoes crystallization on a target plate. A precisely timed laser ionizes the matrix, transferring charges to sample elements, leading to their separation and collection within a vacuum-equipped apparatus. The molecules’ separation is based on the mass/charge ratio, with proteins and biomolecules appearing as distinctive peaks of varying intensities and molecular weights. The intricate results are matched against a comprehensive database of known samples for rapid identification, making MALDI-TOF an essential analytical tool.
Our method employs a stringent threshold standard for result acceptance, ensuring accuracy in species identification. It also suggests close matches and related species. In cases of a “No Match” identification, we offer complimentary DNA Sequencing to aid microbe identification. Our ongoing research and testing of various microorganisms further enrich our database, minimizing the likelihood of “No Match” outcomes.
Optimal accuracy is achieved with fresh growth samples; therefore, we purify and process your samples within 24-48 hours of receipt at our state-of-the-art facility. Rapid report delivery on the same business day ensures timely access to your results.
Harness the speed and efficiency of MALDI-TOF analysis for your samples. Whether it’s detecting potential contaminants or identifying yeast species in your beer or wine, we prioritize meeting your unique needs. MALDI-TOF is just one facet of our commitment to solving contamination and quality challenges.
Learn more about MALDI-TOF testing with Biolog Lab Services here.
