Mitochondrial Function Assays with MitoPlates

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MitoPlate Phenotype

Mitochondrial Function Assays with MitoPlates

MitoPlates™ are cell-based phenotyping assays used to interrogate and characterize mitochondria. They use 96-well plates pre-loaded with tests designed to measure the effects of a diverse set of substrates and inhibitors on mitochondrial function. Any cell type of interest can be added to the plates along with Biolog’s proprietary redox dye. The generation of energy-rich NADH by the cells reduces the redox dye and brings about a color change which can be read and analyzed by Odin™.


MitoPlates can be used to investigate how mitochondria change as a result of processes including

  • Cell differentiation
  • Cancer and aging
  • Neurological disorders
  • Metabolic disorders
  • Immune cell activation arrayed in triplicate,
  • Bacterial/viral infection
  • Innate genetic defects

Certain metabolic substrates rely on different transporters to enter the mitochondria and different dehydrogenases to produce NAD(P)H or FADH2. Mitochondria are also sensitive to specific inhibitors. The MitoPlates consist of two panels, a substrate panel and an inhibitor panel. Together these panels can be used to characterize mitochondria in novel ways, including drug sensitivity, rates of metabolism, and effects of mutations in mitochondria-related genes.

Assay Principles
Checking for Growth with odin

See what’s happening in two ways.

Odin measures a reporter dye over time at one wavelength to measure NADH production, effectively reporting the rate of metabolic respiration.

It also measures optical density (OD) at a second wavelength to determine how quickly the microbes are dividing. Taken together, you get a full picture of how your microbes grow best over time.

If you’re focused on cell growth, just leave the dye out. Odin automatically compares cell growth and metabolic activity curves to provide you with a clearer picture of how fast or slow your microbes are growing.

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