Mitochondrial Function Assays with MitoPlates
MitoPlates are cell-based phenotyping assays used to interrogate and characterize mitochondria. They use 96-well plates pre-loaded with tests designed to measure the effects of a diverse set of substrates and inhibitors on mitochondrial function. Any cell type of interest can be added to the plates along with Biolog’s proprietary redox dye. The generation of energy-rich NADH by the cells reduces the redox dye and brings about a color change which can be read and analyzed by Odin.
MitoPlates can be used to investigate how mitochondria change as a result of processes including
- Cell differentiation
- Cancer and aging
- Neurological disorders
- Metabolic disorders
- Immune cell activation arrayed in triplicate,
- Bacterial/viral infection
- Innate genetic defects
Certain metabolic substrates rely on different transporters to enter the mitochondria and different dehydrogenases to produce NAD(P)H or FADH2. Mitochondria are also sensitive to specific inhibitors. The MitoPlates consist of two panels, a substrate panel and an inhibitor panel. Together these panels can be used to characterize mitochondria in novel ways, including drug sensitivity, rates of metabolism, and effects of mutations in mitochondria-related genes.