In this webinar, Dr. Ian Paulsen, Director, ARC Centre of Excellence in Synthetic Biology, Macquarie University Sydney NSW Australia, introduced solutions to:
- Transcription and TraDIS were used to identify membrane proteins of unknown function that might potentially be new multidrug efflux pumps
- Candidate genes were cloned and expressed in E. coli and Biolog Phenotype MircoArrays™ 11-20 were used to identify putative substrates
- Transport assays were used and we discovered three putative novel drug efflux pump families
- Biochemical and biophysical assays of the purified proteins confirmed their interaction with substrates
