Advanced Phenotypic Analysis

     
 
 
 

Antimicrobial Assays

Phenotype MicroArray (PM) MicroPlate products include a wide spectrum of antimicrobial sensitivity tests. PM MicroPlates PM11-20 are used for sensitivity testing of bacteria, and PM21-25 are used for sensitivity testing of yeasts and fungi. In addition to antibacterials and antifungals, the tests also contain other types of chemicals with antimicrobial activity including metals, detergents, pathway inhibitors, and others. The 360 chemicals predispensed in the PM MicroPlates have been chosen to probe cellular pathways that cannot be tested by metabolic assays. For example, these antimicrobials test for inhibition of transporters, efflux pumps, ribosomes, polymerases, gyrases, membrane function, respiration, and more.

Product Features:

  • 4 well titration series, increasing concentration from left to right
  • Detect relative changes in sensitivity or resistance
  • Use any media as inoculating fluid
  • Test almost all bacteria, yeasts, and fungi

Relevant Research Applications Include:

  • Mechanism of Antibiotic Resistance
    • What do MDR's, nitric oxide, hydrogen sulfide, and proteases have in common? They are all enlisted to resist antibiotics by increasing drug efflux, mitigating oxidative stress, and repairing oxidative damage. See how Phenotype MicroArrays played a role in this type of research.
  • Study of Antibiotic Efflux Transporters
    • Alterations in drug efflux transporters can result in hypersensitivity or resistance to chemicals that may be substrates of the transporter. See how Phenotype MicroArray technology can be used to assay transporter function, assign substrates to transporters, and quickly identify multidrug resistance.
  • Determine Antibiotic Mechanism of Action
    • After an inhibitor is found, an immediate question arises: Is the natural product a novel antimicrobial? Biolog's Phenotype MicroArray (PM) technology offers a unique way to infer the MOA of an inhibitor. See how PM technology can be used to generate high quality isobolograms, which can be scored and clustered to group inhibitors based on this rich fingerprint.
  • Strain Discrimination of Antibiotic Resistance Patterns
    • Antibiotic sensitivity phenotypes serve as an excellent fingerprint for strain discrimination. See how PM antimicrobial assays have been used to discriminate closely related strains for academic study, environmental research, and food safety research.

 

 

 

 

     


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