Biolog Phenotype MicroArray
     Serious Technology for Serious Microbiology February 23, 2010
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Phenotype MicroArrays used in the study of antibiotic mechanisms of resistance.
Recent publications illustrate how Biolog's Phenotype MicroArray (PM) technology has been useful in understanding mechanisms of antibiotic resistance. In this newsletter, we present three papers from the primary literature and two news highlights.
 Endogenous nitric oxide protects bacteria against a wide spectrum of antibiotics.
Phenotype MicroArray testing allowed the authors to determine that deletion of bacterial nitric oxide synthase results in increased sensitivity to a broad spectrum of antibiotics. Further study indicates that nitric oxide modifies toxic chemicals and relieves oxidative stress caused by antibiotics.

The abstract can be viewed here:

 Phenotypic changes in ciprofloxacin-resistant Staphylococcus aureus.
PM technology was used to characterize two ciprofloxacin target mutants. No alterations in metabolism were observed, but several antibiotic sensitivity phenotypes are discussed.

The abstract can be viewed here:

 Fitness costs and stability of a high-level ciprofloxacin resistance phenotype in Salmonella enterica serotype enteritidis: reduced infectivity associated with decreased expression of Salmonella pathogenicity island 1 genes.
Phenotype MicroArrays were used to characterize pH and osmotic susceptibilities of ciprofloxacin resistant mutants.

The abstract can be viewed here:

 When it comes to antibiotics, bacteria show some NO-how.
A research highlight from the Journal of Molecular Biology

Download a copy of the article here:

 A specific protein, possibly NO enable D. radiodurans to cope with UV
Current topic from Microbe Magazine

The article can be viewed here:

Phenotype MicroArray Technology
Biolog's Phentoype MicroArray technology enables researchers to evaluate nearly 2000 phenotypes of a microbial cell in a single experiment. This integrated system of cellular assays, instrumentation and bioinformatics software provides cellular knowledge that complements molecular information, helping you interpret and find the relevant aspects in massive amounts of gene expression or proteomics data. Through comprehensive and precise quantitation of phenotypes, researchers are able to obtain an unbiased perspective of the effect on cells of genetic differences, environmental change, exposure to chemicals or drugs, and more.
Applications for Phenotype MicroArrays
Phenotype MicroArrays add a fresh cell-based perspective to a wide range of activities.

  • Discovery research
  • Cell line characterization
  • Mutant and mutation characterization
  • Determination of gene function
  • Culture medium optimization
  • BioProcess development and improvement
  • BioProcess QC and validation
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