CA - November 29, 2004 - Biolog, Inc. announced today that it will use its innovative Phenotype MicroArray™ (PM) technology to begin broad scale phenotyping of the E. coli genome which consists of more than 4,000 genes. Phenotype MicroArrays represent a fundamental technology platform, which allows scientists to easily and efficiently test hundreds to thousands of cellular traits simultaneously. The technology has the promise of determining cellular phenotypic expression in many areas of research. The two most important near term applications are using PMs to determine the effect of genetic changes on cells and to determine the effect of drugs on cells.
Using the E. coli K-12 collection developed at the Nara Institute, Biolog will phenotype each gene knock-out and develop a database of phenotypes for all genes. This work will begin at Biolog’s facility in Hayward , CA and the first 100 genes will be analyzed by March 2005. The data from those 100 strains will be reviewed by scientists from Biolog, Inc. and the Nara Institute in Nara , Japan . The information from this work will be organized and cataloged by Axiohelix of Tokyo, Japan. It is hoped that the phenotypic analysis will then be expanded in 2005 to include the entire E. coli genome.
Tim Mullane , President & CEO of Biolog stated that, “E. coli is one of the most studied model cell lines in the world and despite the early mapping of this organism’s genome, many genes have no known function. Even where function is known, this information is often incomplete. We have already performed a significant amount of work with researchers focused on E. coli over the last two years, and now we will be involved in systematically exploring the entire genome with our entire array set to provide valuable insight into each gene’s function. The potential value of this comprehensive database of information to researchers in anti-bacterial development, biotechnology, and fermentation sciences is enormous. We hope the knowledge gained will lead to more rapid advances in multiple areas.”
Biolog, a privately held company based in Hayward , CA , is a pioneer in the development of powerful new cell analysis tools for solving critical problems in clinical, pharmaceutical, and biotechnology research and development. The company’s Phenotype MicroArray technology and OmniLog ® PM System can be used in the discovery and development of new drugs as well as bioactive agents for animal and plant applications. Further information can be obtained at the company’s website, www.biolog.com.
The Nara Institute of Science and Technology is a national university comprised solely of graduate schools. NAIST strives to promote basic research in advanced science and technology and to educate individuals to pursue R&D in the multiple areas of science, see (www.naist.jp).
Axiohelix is a privately held company based in Tokyo , Japan serving multinational clients. The company offers a suite of services, which include: consultancy, e-business and pervasive computing solutions, system integration, digital design, and outsourced code production. The company’s goal is to combine extensive industry experience, best practices in software production, with an efficient cost structure to their clients, see (www.axiohelix.com).